Recombinant:Article Title: Cellular and immune adaptations at the maternal-fetal interface in bats.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Pan-Cytokeratin (pan-KRT) Abcam ab308262;RRID:AB_3676255 Vimentin Abcam ab92547;RRID:AB_10562134 KRT18 (CK18) Abcam ab668;RRID:AB_305647 CD45 Abcam ab10558;RRID:AB_442810 CD68 Abcam ab125212;RRID:AB_10975465 CD3 Abcam ab16669;RRID:AB_443425 F4/80 Invitrogen 14-4801-82;RRID:AB_467558 Ki67 Abcam ab15580;RRID:AB_443209 SOX9 Abcam ab185230;RRID:AB_2715497 KRT7 Abcam ab181598;RRID:AB_2783822 GATA3 Cell Signaling Technology (CST) 5852;RRID:AB_10835690 NCAM-1 Abcam ab9018;RRID:AB_306945 CD34 Abcam ab81289;RRID:AB_1640331 Claudin-5 Invitrogen 35–2500;RRID:AB_87321 CD163 Abcam ab182422;RRID:AB_2753196 SOX17 Abcam Ab224637;RRID:AB_2801385 Goat anti-mouse IgG, CoraLite Plus 488 (Multi-rAb) Proteintech RGAM002;RRID:AB_3068538 Goat anti-rabbit IgG, CoraLite Plus 594 (Multi-rAb) Proteintech RGAR004;RRID:AB_3073502 Goat anti-mouse IgG, Alexa Fluor 488 Invitrogen A-11001;RRID:AB_2534069 Goat anti-mouse IgG, Alexa Fluor 594 Invitrogen A-11005;RRID:AB_2534073 Goat anti-rabbit IgG, Alexa Fluor 488 Invitrogen A-11008;RRID:AB_143165 Goat anti-rabbit IgG, Alexa Fluor 694 Invitrogen A-11012;RRID:AB_2534079 Phalloidin, Alexa Fluor 594 Invitrogen A12381 Phalloidin, Alexa Fluor 647 Invitrogen A22287 Biological samples Jamaican fruit bat placentas (pregnant females) Jamaican fruit bat placentas (pregnant females) See Table S12 Mouse placentas (C57BL/6J) Mouse placentas (C57BL/6J) The Jackson Laboratory #000664 Human Trophoblast Organoids Cryopreserved samples organoid lines from previous studies Yang et al. 39 Chemicals, peptides, and recombinant proteins Trypsin-250 (0.2%) Alfa Aesar J63993-09 EDTA (0.02%) Sigma-Aldrich E9884-100G Collagenase V (1.0 mg/mL) Sigma-Aldrich C9263-100MG Dispase II (1.25 U/mL) Sigma-Aldrich D4693 Collagenase V (0.4 mg/mL) Sigma-Aldrich C-9263 RBC Lysis Buffer (1×) Invitrogen 00433357 Advanced DMEM/F12 Life Technologies 12634–010 Growth Factor Reduced Matrigel Corning 356231 24-well tissue culture plate Corning 3526 B27 Supplement (1×) Life Technologies 17504–044 N2 Supplement (1×) Life Technologies 17502–048 Fetal Bovine Serum (FBS), 10% Cytiva HyClone SH30070.03 GlutaMAX Supplement, 2 mM Life Technologies 35050–061 (Continued on next page) 18 Cell Reports 44, 116645, December 23, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Primocin, 100 μg/mL InvivoGen ant-pm-1 N-Acetyl-L-cysteine, 1.25 mM Sigma A9165 A83-01, 500 nM Tocris 2939 CHIR99021, 1.5 μM Tocris 4423 EGF, recombinant human, 50 ng/mL Gibco PHG0314 R-spondin 1, recombinant human, 80 ng/mL R&D Systems 4645-RS-100 Noggin, recombinant human, 100 ng/mL Peprotech 120-10C FGF2, recombinant human, 100 ng/mL Peprotech 100-18C FGF10, recombinant human, 100 ng/mL Peprotech 100–26 HGF, recombinant human, 50 ng/mL Peprotech 100–39 Nicotinamide, 10 mM Sigma N0636-100G Y-27632, 5 μM Sigma Y0503-1MG Prostaglandin E2 (PGE2), 2.5 μM R&D Systems 22-961-0 TrypLE Express Gibco 12605–028 TrypLE Express ThermoFisher Scientific 12605010 Cell Recovery Solution Corning 354253 DMEM (fibroblast media) Corning 10017CV FBS (for fibroblast media) Gibco A56707 Penicillin/Streptomycin (1×) Gibco 15140 Non-essential amino acids (NEAA), 1× Cytiva SH30238.01 Sodium Pyruvate, 1 mM Cytiva SH30239.01 HEPES, 10 mM Gibco 15630–106 Penicillin/Streptomycin (Lonza) Lonza 17-602E 100 μm Cell Strainer VWR 732–2759 Mini cryomolds (7 × 7 × 5 mm) Simport Scientific M475 Microscope slides Fisherbrand 1255015 Flowmi Cell Strainers, 40 μm Bel-Art H13680-0040 Deposited data snRNA-seq datasets This study PRJNA1251203; PRJNA1251235 Bulk RNA-seq (poly I:C vs. mock) This study PRJNA1251670 Publicly available datasets used in this study Human first-trimester snRNA-Seq dataset Wang et al. 20 PRJNA1035951 Mouse snRNA-Seq dataset 1 Lopez-Tello et al. 35 PRJNA954811 Mouse snRNA-Seq dataset 2 Guo et al. 36 PRJNA781579 Critical commercial assays and instruments Keyence BZ-X810 microscope + motorized XY Keyence BZ-X810 Keyence BZ-X800 Analysis Software Keyence v1.1.30.19 Olympus Fluoview 3000 inverted confocal Olympus FV3000 EVOS FL fluorescence microscope Thermo Fisher Scientific EVOS FL Grant shaking water bath Grant Instruments LSB12US Agilent TapeStation System Agilent 4200 Cellometer Ascend Automated Cell Counter Revvity Ascend Chromium Reagent Kit v4 10x Genomics v4 (Kit) NovaSeq X Plus Illumina Instrument Software and algorithms Keyence BZ-X800 Analysis (v1.1.30.19) N/A https://www.keyence.com/landing/ microscope/lp_fluorescence.jsp Cell Ranger 10x Genomics v6.1.2 Seurat Satija Lab v5.0.1 (Continued on next page) Cell Reports 44, 116645, December 23, 2025 19
Red Blood Cell Lysis:Article Title: Cellular and immune adaptations at the maternal-fetal interface in bats.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Pan-Cytokeratin (pan-KRT) Abcam ab308262;RRID:AB_3676255 Vimentin Abcam ab92547;RRID:AB_10562134 KRT18 (CK18) Abcam ab668;RRID:AB_305647 CD45 Abcam ab10558;RRID:AB_442810 CD68 Abcam ab125212;RRID:AB_10975465 CD3 Abcam ab16669;RRID:AB_443425 F4/80 Invitrogen 14-4801-82;RRID:AB_467558 Ki67 Abcam ab15580;RRID:AB_443209 SOX9 Abcam ab185230;RRID:AB_2715497 KRT7 Abcam ab181598;RRID:AB_2783822 GATA3 Cell Signaling Technology (CST) 5852;RRID:AB_10835690 NCAM-1 Abcam ab9018;RRID:AB_306945 CD34 Abcam ab81289;RRID:AB_1640331 Claudin-5 Invitrogen 35–2500;RRID:AB_87321 CD163 Abcam ab182422;RRID:AB_2753196 SOX17 Abcam Ab224637;RRID:AB_2801385 Goat anti-mouse IgG, CoraLite Plus 488 (Multi-rAb) Proteintech RGAM002;RRID:AB_3068538 Goat anti-rabbit IgG, CoraLite Plus 594 (Multi-rAb) Proteintech RGAR004;RRID:AB_3073502 Goat anti-mouse IgG, Alexa Fluor 488 Invitrogen A-11001;RRID:AB_2534069 Goat anti-mouse IgG, Alexa Fluor 594 Invitrogen A-11005;RRID:AB_2534073 Goat anti-rabbit IgG, Alexa Fluor 488 Invitrogen A-11008;RRID:AB_143165 Goat anti-rabbit IgG, Alexa Fluor 694 Invitrogen A-11012;RRID:AB_2534079 Phalloidin, Alexa Fluor 594 Invitrogen A12381 Phalloidin, Alexa Fluor 647 Invitrogen A22287 Biological samples Jamaican fruit bat placentas (pregnant females) Jamaican fruit bat placentas (pregnant females) See Table S12 Mouse placentas (C57BL/6J) Mouse placentas (C57BL/6J) The Jackson Laboratory #000664 Human Trophoblast Organoids Cryopreserved samples organoid lines from previous studies Yang et al. 39 Chemicals, peptides, and recombinant proteins Trypsin-250 (0.2%) Alfa Aesar J63993-09 EDTA (0.02%) Sigma-Aldrich E9884-100G Collagenase V (1.0 mg/mL) Sigma-Aldrich C9263-100MG Dispase II (1.25 U/mL) Sigma-Aldrich D4693 Collagenase V (0.4 mg/mL) Sigma-Aldrich C-9263 RBC Lysis Buffer (1×) Invitrogen 00433357 Advanced DMEM/F12 Life Technologies 12634–010 Growth Factor Reduced Matrigel Corning 356231 24-well tissue culture plate Corning 3526 B27 Supplement (1×) Life Technologies 17504–044 N2 Supplement (1×) Life Technologies 17502–048 Fetal Bovine Serum (FBS), 10% Cytiva HyClone SH30070.03 GlutaMAX Supplement, 2 mM Life Technologies 35050–061 (Continued on next page) 18 Cell Reports 44, 116645, December 23, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Primocin, 100 μg/mL InvivoGen ant-pm-1 N-Acetyl-L-cysteine, 1.25 mM Sigma A9165 A83-01, 500 nM Tocris 2939 CHIR99021, 1.5 μM Tocris 4423 EGF, recombinant human, 50 ng/mL Gibco PHG0314 R-spondin 1, recombinant human, 80 ng/mL R&D Systems 4645-RS-100 Noggin, recombinant human, 100 ng/mL Peprotech 120-10C FGF2, recombinant human, 100 ng/mL Peprotech 100-18C FGF10, recombinant human, 100 ng/mL Peprotech 100–26 HGF, recombinant human, 50 ng/mL Peprotech 100–39 Nicotinamide, 10 mM Sigma N0636-100G Y-27632, 5 μM Sigma Y0503-1MG Prostaglandin E2 (PGE2), 2.5 μM R&D Systems 22-961-0 TrypLE Express Gibco 12605–028 TrypLE Express ThermoFisher Scientific 12605010 Cell Recovery Solution Corning 354253 DMEM (fibroblast media) Corning 10017CV FBS (for fibroblast media) Gibco A56707 Penicillin/Streptomycin (1×) Gibco 15140 Non-essential amino acids (NEAA), 1× Cytiva SH30238.01 Sodium Pyruvate, 1 mM Cytiva SH30239.01 HEPES, 10 mM Gibco 15630–106 Penicillin/Streptomycin (Lonza) Lonza 17-602E 100 μm Cell Strainer VWR 732–2759 Mini cryomolds (7 × 7 × 5 mm) Simport Scientific M475 Microscope slides Fisherbrand 1255015 Flowmi Cell Strainers, 40 μm Bel-Art H13680-0040 Deposited data snRNA-seq datasets This study PRJNA1251203; PRJNA1251235 Bulk RNA-seq (poly I:C vs. mock) This study PRJNA1251670 Publicly available datasets used in this study Human first-trimester snRNA-Seq dataset Wang et al. 20 PRJNA1035951 Mouse snRNA-Seq dataset 1 Lopez-Tello et al. 35 PRJNA954811 Mouse snRNA-Seq dataset 2 Guo et al. 36 PRJNA781579 Critical commercial assays and instruments Keyence BZ-X810 microscope + motorized XY Keyence BZ-X810 Keyence BZ-X800 Analysis Software Keyence v1.1.30.19 Olympus Fluoview 3000 inverted confocal Olympus FV3000 EVOS FL fluorescence microscope Thermo Fisher Scientific EVOS FL Grant shaking water bath Grant Instruments LSB12US Agilent TapeStation System Agilent 4200 Cellometer Ascend Automated Cell Counter Revvity Ascend Chromium Reagent Kit v4 10x Genomics v4 (Kit) NovaSeq X Plus Illumina Instrument Software and algorithms Keyence BZ-X800 Analysis (v1.1.30.19) N/A https://www.keyence.com/landing/ microscope/lp_fluorescence.jsp Cell Ranger 10x Genomics v6.1.2 Seurat Satija Lab v5.0.1 (Continued on next page) Cell Reports 44, 116645, December 23, 2025 19
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